PHEREC Technical
Memorandum # 11
DILUTION
OF SCOURGE® FOR THE BOTTLE BIOASSAY
According to the
manufacturer’s label 1 liter of SCOURGE® contains 36 grams of resmethrin and 144 grams of piperonyl butoxide.
1 liter of
SCOURGE® contains 36 grams of resmethrin.
1 milliliter of SCOURGE® contains 36 milligrams of resmethrin.
TAKE one (1)
milliliter of SCOURGE® and place it in a 100 ml volumetric flask. Diulute it to 100 ml
FINAL VOLUME with A.C.S. acetone [A.C.S. acetone is the grade of acetone approved by the
American Chemical Society.] This is the STOCK
solution.
The STOCK
solution contains 0.36 milligrams per ml.
TAKE 8.3 ml of
the STOCK solution and place it in a volumetric flask.
Dilute it to 100 ml FINAL VOLUME with A.C.S. acetone. This is the TEST solution.
The TEST solution contains 0.0299 mg of resmethrin per ml.
The TEST solution contains approximately 30 micrograms of AI per ml.
USE 1 ml of the
TEST solution in each bottle of the bioassay.
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Complete
instructions for the procedure for calculating time-mortality curves by the
bottle bioassay are available at http://www.pherec.org/memoranda/procedure.html
For more
information, CONTACT Dr. Jack Petersen at (850) 872-4184 extension 36 or email drjack3@hotmail.com
Please check our
Web site for updates on this protocol: http://www.pherec.org
This Technical
Memorandum was drafted in response to an inquiry from Jena Moore at East Volusia Mosquito
Control District., April 2006.