DILUTION OF PERMANONE® 10% EC FOR THE BOTTLE BIOASSAY

DRAFT DOCUMENT FOR REVIEW

PHEREC Technical Memorandum # 15

Start with the label.  According to the label:

1 liter of PERMANONE® 10% EC contains 100 grams of permethrin

1 liter of PERMANONE® 10% EC contains 100 grams of permethrin.
1 milliliter of  PERMANONE® 10% EC contains 0.1 grams of permethrin.
1 milliliter of  PERMANONE® 10% EC contains 100 milligrams of permethrin.

TAKE one (1) milliliter of PERMANONE® 10% EC   and, using a volumetric flask, dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.   [A.C.S. acetone is the grade of acetone approved by the American Chemical Society.]  This is the STOCK solution.

The STOCK solution contains  1.00 milligrams/ml

TAKE 4.3 ml of the STOCK solution and dilute it to 100 ml FINAL VOLUME with A.C.S. acetone.  This is the TEST solution.

This is equivalent to 43 micrograms/ml

The TEST solution contains 0.043 milligrams/ml permethrin
The TEST solution contains 43 micrograms/ml permethrin

USE 1 ml of the TEST solution in each bottle of the bioassay according to the standard protocol.

 

For more information, CONTACT Dr. Jack Petersen
at (850) 872-4184 extension 36 or email drjack3@hotmail.com

Please check our Web site for updates on this protocol:
http://pherec.org

Edited: March 25, 2008 jlp