DILUTION OF PERMANONE® 10% EC FOR THE BOTTLE BIOASSAY
DRAFT DOCUMENT FOR REVIEW
PHEREC Technical Memorandum # 15
Start with the label. According to the label:
1 liter of PERMANONE® 10% EC contains 100 grams of permethrin
1 liter of PERMANONE® 10% EC contains 100 grams of permethrin.
1 milliliter of PERMANONE® 10% EC contains 0.1 grams of permethrin.
1 milliliter of PERMANONE® 10% EC contains 100 milligrams of permethrin.
TAKE one (1) milliliter of PERMANONE® 10% EC and, using a volumetric flask, dilute it to 100 ml FINAL VOLUME with A.C.S. acetone. [A.C.S. acetone is the grade of acetone approved by the American Chemical Society.] This is the STOCK solution.
The STOCK solution contains 1.00 milligrams/ml
TAKE 4.3 ml of the STOCK solution and dilute it to 100 ml FINAL VOLUME with A.C.S. acetone. This is the TEST solution.
This is equivalent to 43 micrograms/ml
The TEST solution contains 0.043 milligrams/ml permethrin
The TEST solution contains 43 micrograms/ml permethrin
USE 1 ml of the TEST solution in each bottle of the bioassay according to the standard protocol.
For more information, CONTACT Dr. Jack Petersen
at (850) 872-4184 extension 36 or email drjack3@hotmail.com
Please check our Web site for updates on this protocol:
http://pherec.org
Edited: March 25, 2008 jlp